Open AccessChemical stress‐responsive genes from the lignin‐degrading fungus Phanerochaete chrysosporium exposed to dibenzo‐ p ‐dioxin
Author(s) -
Kurihara Hiroyuki,
Wariishi Hiroyuki,
Tanaka Hiroo
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11269.x
Subject(s) - phanerochaete , biochemistry , chrysosporium , chemistry , lignin peroxidase , cellobiose dehydrogenase , manganese peroxidase , enzyme , peroxidase , biology , microbiology and biotechnology , cellulase , cellobiose
The stress‐responsive genes expressed against the exogenous addition of dibenzo‐ p ‐dioxin from the lignin‐degrading basidiomycete, Phanerochaete chrysosporium , were determined utilizing a differential display reverse transcription‐PCR technique. Six cDNA fragments, exhibiting a high homology with various proteins from other microorganisms, were identified via a BLAST search; that is, NADH‐ubiquinone oxidoreductase (NUO), ATP/ADP carrier, uric acid‐xanthine permease, manganese superoxide dismutase, 3‐hydroxybutyryl‐coenzyme A dehydrogenase, and cytoskeletal protein. The expression of NUO was also up‐regulated by catechol and trihydroxybenzene but not by dibenzofuran, suggesting that NUO expression was initiated by the formation of quinone products through the reaction of extracellular one‐electron oxidizing enzymes with dibenzo‐ p ‐dioxin.