Open AccessControl of autogenous activation of Herbaspirillum seropedicae nifA promoter by the IHF protein
Author(s) -
Wassem Roseli,
Pedrosa Fábio O,
Yates Marshall G,
Rego Fabiane G.M,
Chubatsu Leda S,
Rigo Liu U,
Souza Emanuel M
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11263.x
Subject(s) - escherichia coli , strain (injury) , promoter , microbiology and biotechnology , chemistry , biology , gene , genetics , gene expression , anatomy
Analysis of the expression of the Herbaspirillum seropedicae nifA promoter in Escherichia coli and Herbaspirillum seropedicae , showed that nifA expression is primarily dependent on NtrC but also required NifA for maximal expression under nitrogen‐fixing conditions. Deletion of the IHF (integration host factor)‐binding site produced a promoter with two‐fold higher activity than the native promoter in the H. seropedicae wild‐type strain but not in a nifA strain, indicating that IHF controls NifA auto‐activation. IHF is apparently required to prevent overexpression of the NifA protein via auto‐activation under nitrogen‐fixing conditions in H. seropedicae .