Open AccessA molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita
Author(s) -
Yokoyama Kazuhira,
Tateishi Takahiro,
Marumoto Takuya,
Saito Masanori
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11262.x
Subject(s) - fungus , biology , polymerase chain reaction , microbiology and biotechnology , dna sequencing , spore , strain (injury) , sequence (biology) , dna , botany , genetics , gene , anatomy
To investigate the auto‐ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnostic sequence from both DNA preparations of single spores and colonized roots. This protocol enabled us to distinguish the isolate among several isolates from Japan, Nepal and the USA.