Open AccessPolyhydroxyalkanoate biosynthesis in Pseudomonas pseudoalcaligenes YS1
Author(s) -
Hang Xiaoming,
Lin Zhaoxing,
Chen Jingyu,
Wang Guanlin,
Hong Kui,
Chen GuoQiang
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11247.x
Subject(s) - polyhydroxyalkanoates , polyhydroxybutyrate , mutant , escherichia coli , biosynthesis , chemistry , microbiology and biotechnology , biology , biochemistry , pseudomonadaceae , pseudomonas , bacteria , gene , genetics
Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium‐chain‐length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B ( fadB :: Tet ) was constructed to express the cloned PHA synthesis gene phaC1 Pp or phaC2 Pp . The fadB deleted mutant KM32B harboring phaC1 Pp or phaC2 Pp showed mcl PHA accumulation while the intact E. coli KM32 did not. The results demonstrated that P. pseudoalcaligenes YS1 possessed at least two PHA synthesis pathways; one of them was responsible for production of mcl PHA.