Open AccessInduction of an outer membrane protein of 78 kDa in Vibrio vulnificus cultured in the presence of desferrioxamine B under iron‐limiting conditions
Author(s) -
Aso Hiroaki,
Miyoshi Shinichi,
Nakao Hiroshi,
Okamoto Keinosuke,
Yamamoto Shigeo
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11246.x
Subject(s) - limiting , bacterial outer membrane , chemistry , biophysics , biochemistry , biology , escherichia coli , gene , mechanical engineering , engineering
Although Vibrio vulnificus is known to be able to utilize ferrioxamine B as an iron source, its outer membrane receptor remains to be determined. In this study, we found that V. vulnificus expressed a new outer membrane protein of 78 kDa when grown in the presence of desferrioxamine B under iron‐limiting conditions. The desferrioxamine B‐dependent iron uptake was only observed in bacterial cells expressing this protein. Furthermore, non‐denaturing polyacrylamide gel electrophoresis followed by autoradiography of the outer membrane preparation containing the 78‐kDa protein preincubated with [ 55 Fe]ferrioxamine B provided a single radioactive band in which the 78‐kDa outer membrane protein was present as the major component. These lines of evidence suggest that the inducible 78‐kDa protein may serve as the cell‐surface receptor for ferrioxamine B.