Open AccessTwo immunologically distinct types of protofilaments can be identified in Natrialba magadii flagella
Author(s) -
Pyatibratov Michael G,
Leonard Kevin,
Tarasov Valery Y,
Fedorov Oleg V
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11239.x
Subject(s) - immunogold labelling , flagellin , flagellum , polyclonal antibodies , crystallography , biology , electron microscope , chemistry , biophysics , physics , ultrastructure , antibody , bacteria , genetics , anatomy , optics
We examine distribution of flagellins along multicomponent flagellar filaments (FF) and protofilaments (PF) of the haloalkaliphilic archaeon Natrialba magadii using immunogold electron microscopy. A high specific polyclonal antibody raised to one of the flagellin types bound homogeneously to the undissociated FF along the full length. At the same time both uniformly labelled and completely unlabelled PF, outwardly indistinguishable one from another, were observed.