Open AccessEscherichia coli genes involved in resistance to pyrazinoic acid, the active component of the tuberculosis drug pyrazinamide
Author(s) -
Schaller Alain,
Guo Ming,
Gisanrin Oluwatosin,
Zhang Ying
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11235.x
Subject(s) - complementation , escherichia coli , mutant , biology , efflux , pyrazinamide , genetics , gene , microbiology and biotechnology , antibiotics , rifampicin
The natural resistance of Escherichia coli to pyrazinoic acid (POA), the active derivative of pyrazinamide, was investigated. The TolC mutant was found to be more susceptible to POA and other weak acids than the wild‐type strain. Mutation in EmrB but not AcrB efflux protein slightly increased POA susceptibility. Two transposon mutants with increased susceptibility to POA were found to harbor mutations in acnA encoding aconitase‐1 and ygiY encoding a putative two‐component sensor protein. Complementation of the AcnA and YgiY mutants conferred resistance to POA, whereas the complemented TolC mutant became resistant to POA and other weak acids.