Open AccessTransformation of the edible mushroom Pleurotus ostreatus by particle bombardment
Author(s) -
Sunagawa Masahide,
Magae Yumi
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11216.x
Subject(s) - pleurotus ostreatus , auxotrophy , transformation (genetics) , mushroom , chemistry , dna , plasmid , particle (ecology) , genomic dna , biology , biochemistry , food science , gene , escherichia coli , ecology
Uracil auxotroph of Pleurotus ostreatus was transformed to prototrophy by means of particle bombardment. Five transformants were obtained under three conditions differing in the two parameters of target distance and helium pressure. The transformation frequency was one transformant per μg of DNA. In the transformants, plasmid DNAs were integrated into the genomic DNA and stably maintained. This is the first report on transformation of P. ostreatus by particle bombardment.