Open AccessConstruction and intergeneric conjugative transfer of a pSG5‐based cosmid vector from Escherichia coli to the polyisoprene rubber degrading strain Micromonospora aurantiaca W2b
Author(s) -
Rose Karsten,
Steinbüchel Alexander
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11214.x
Subject(s) - shuttle vector , cosmid , escherichia coli , cloning vector , strain (injury) , biology , cloning (programming) , streptomyces , mutant , microbiology and biotechnology , molecular cloning , vector (molecular biology) , genetics , bacteria , gene , anatomy , computer science , peptide sequence , recombinant dna , programming language
A non‐rubber degrading mutant of the polyisoprene rubber degrading bacterium Micromonospora aurantiaca W2b lacking the capability to form halos on latex overlay agar plates was isolated after N ‐methyl‐ N ‐nitro‐ N ‐nitrosoguanidine mutagenesis. A 10.3‐kb shuttle cosmid vector pGM446 was constructed from the Streptomyces cloning vectors pGM160 and pOJ446. This vector was transferred by conjugation from Escherichia coli to M. aurantiaca W2b. The frequency of formation of exconjugants with pGM446 was 3.6×10 −3 . This vector could be useful for shotgun cloning of genes into the non‐rubber degrading mutant L1 from M. aurantiaca W2b.