Open AccessCharacterisation of the QM gene of Trypanosoma brucei
Author(s) -
Lillico Simon G,
Mottram Jeremy C,
Murphy Noel B,
Welburn Susan C
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11213.x
Subject(s) - trypanosoma brucei , biology , saccharomyces cerevisiae , endoplasmic reticulum , gene , genetics , ribosome , drosophila melanogaster , microbiology and biotechnology , rna
The QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of Trypanosoma brucei and determined its sequence. Alignment with QM sequences from Saccharomyces cerevisiae , Arabidopsis thaliana , Drosophila melanogaster and Homo sapiens revealed greater than 60% identity. Southern blot analysis revealed multiple copies of QM within the trypanosome genome. An epitope tag was inserted into the C‐terminus of the T. brucei QM and the protein expressed under inducible control in procyclic form trypanosomes. Immune fluorescence microscopy revealed co‐localisation with the GPI:protein transamidase component, GPI8, a distribution indicative of ribosome association with the rough endoplasmic reticulum.