Open AccessA molecular approach for early monitoring of decay basidiomycetes in wood chips
Author(s) -
Adair Solveig,
Kim Seong Hwan,
Breuil Colette
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11212.x
Subject(s) - restriction fragment length polymorphism , biology , spacer dna , ribosomal dna , primer (cosmetics) , basidiomycota , dead wood , terminal restriction fragment length polymorphism , dna , polymerase chain reaction , botany , genetics , gene , phylogenetics , chemistry , ecology , biodiversity , organic chemistry
A DNA‐based method for the early detection and identification of decay basidiomycetes on wood chips is described. From 26 species of wood‐decaying basidiomycetes and 20 species of wood‐inhabiting ascomycetes, DNA fragments containing the internal transcribed spacers, 5.8S and partial 28S rDNA were amplified by PCR using the ITS1‐F–NL2 rDNA primer pair and analysed through sequencing and restriction digestion. A decay basidiomycete‐specific restriction fragment length polymorphism (RFLP) pattern was generated by Dra I. Using this PCR‐RFLP method the detection of decay fungi is possible 4 days after the inoculation of the wood chips with either a single culture or mixed species.