Open AccessA broad host range plasmid vector that does not encode replication proteins
Author(s) -
Kalyaeva Eza,
Bass Irina,
Kholodii Gennady,
Nikiforov Vadim
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11208.x
Subject(s) - replicon , plasmid , biology , acinetobacter calcoaceticus , alcaligenes , escherichia coli , autonomously replicating sequence , genetics , origin of replication , dna , dna replication , microbiology and biotechnology , bacteria , gene , acinetobacter , pseudomonas
The 640‐bp minimal replication region derived from a plasmid DNA preparation from an Acidothiobacillus ferrooxidans strain capable of autonomous replication in a range of Gram‐negative bacteria ( Escherichia coli , Pseudomonas aeruginosa , Acinetobacter calcoaceticus and Alcaligenes faecalis ) was identified. This DNA fragment (named TFK replicon) does not encode Rep proteins and appears to be unrelated to other known replicons.