Open AccessGlutamate dehydrogenase of Halobacterium salinarum : evidence that the gene sequence currently assigned to the NADP + ‐dependent enzyme is in fact that of the NAD + ‐dependent glutamate dehydrogenase
Author(s) -
Hayden Bronagh M.,
Bonete MaríaJosé,
Brown Paul E.,
Moir Arthur J.G.,
Engel Paul C.
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11200.x
Subject(s) - glutamate dehydrogenase , nad+ kinase , biology , halobacterium salinarum , biochemistry , dehydrogenase , glutamate receptor , gene , enzyme , microbiology and biotechnology , archaea , receptor
A GDH gene from Halobacterium salinarum has been cloned and sequenced and the publication assigns the sequence to the NADP + ‐glutamate dehydrogenase of this organism. We have expressed this gene in Escherichia coli and find that it encodes an NAD + ‐dependent glutamate dehydrogenase without activity towards NADP + . Further, peptide sequence from the two corresponding proteins supports the view that the deposited sequence is indeed that of the NAD + ‐dependent glutamate dehydrogenase. Sequence from the NAD + ‐dependent protein matches the published gene sequence, whereas sequence from the NADP + glutamate dehydrogenase does not.