Open AccessOptimization of a rapid and sensitive identification system for Salmonella enteritidis by capillary electrophoresis with laser‐induced fluorescence
Author(s) -
Shintani Tomoyoshi,
Yamada Kazutaka,
Torimura Masaki
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11188.x
Subject(s) - salmonella enteritidis , capillary electrophoresis , polyclonal antibodies , fluorescence , nucleic acid , chemistry , chromatography , stain , laser induced fluorescence , salmonella , staining , bacteria , biochemistry , biology , antibody , optics , genetics , physics , immunology
A system coupling capillary electrophoresis (CE) with a laser‐induced fluorescence (LIF) system for the identification of Salmonella enteritidis was developed. Addition of an appropriate amount of sodium alginate and NaCl to the running buffer made it possible to obtain a reproducible sharp peak. Two fluorescent staining methods using a cell‐permeable nucleic acid stain and a salmonellae‐specific polyclonal antibody were adapted to the system. The CE–LIF successfully detected as few as three cells per injection from a pure culture of S. enteritidis . The CE–LIF system can be conveniently used for rapid and highly sensitive identification of S. enteritidis .