Open AccessChromosomal ampC genes in Enterobacter species other than Enterobacter cloacae , and ancestral association of the ACT‐1 plasmid‐encoded cephalosporinase to Enterobacter asburiae
Author(s) -
Rottman Martin,
Benzerara Yahia,
HanauBerçot Béatrice,
Bizet Chantal,
Philippon Alain,
Arlet Guillaume
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11164.x
Subject(s) - enterobacter cloacae , enterobacter , biology , plasmid , enterobacteriaceae , microbiology and biotechnology , escherichia coli , gene , genetics
The amplification and sequence of ampC genes in Enterobacter asburiae , Enterobacter cancerogenus , Enterobacter dissolvens , Enterobacter hormaechei and Enterobacter intermedius bring the number of known cephalosporinase sequences from the genus Enterobacter to seven. Expression in Escherichia coli of the ampC genes from E. asburiae , E. hormaechei and E. intermedius established the functional nature of these genes. ampC from E. asburiae shows 96.5% identity to bla ACT‐1 encoding a plasmid‐borne cephalosporinase previously believed to derive from Enterobacter cloacae . The reassignment of ACT‐1 ancestry to E. asburiae is confirmed by the 95.5% identity between ampR upstream of bla ACT‐1 and ampR from E. asburiae .