Open AccessArchitectural requirements for optimal activation by tandem CRP molecules at a class I CRP‐dependent promoter
Author(s) -
Tebbutt John,
Rhodius Virgil A,
Webster Christine L,
Busby Stephen J.W
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11159.x
Subject(s) - promoter , rna polymerase , transcription (linguistics) , microbiology and biotechnology , protein subunit , polymerase , biology , dna , chemistry , rna , genetics , gene , gene expression , linguistics , philosophy
The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C‐terminal domain of the RNA polymerase α subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position −61.5 and the other site located at different upstream positions. Optimal CRP‐dependent activation of transcription is observed when the upstream DNA site for CRP is located at position −93.5 or at position −103.5. Evidence is presented to suggest that activation by the upstream‐bound CRP molecule is due to interaction with the C‐terminal domain of the RNA polymerase α subunit.