Open AccessReductive deamination as a new step in the anaerobic microbial degradation of halogenated anilines
Author(s) -
Travkin Vasili,
Baskunov Boris P.,
Golovlev Eugene L.,
Boersma Marelle G.,
Boeren Sjef,
Vervoort Jacques,
Berkel Willem J.H.,
Rietjens Ivonne M.C.M.,
Golovleva Ludmila A.
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11149.x
Subject(s) - deamination , chemistry , reductive dechlorination , oxidative deamination , enrichment culture , degradation (telecommunications) , organic chemistry , microbial biodegradation , biodegradation , microorganism , bacteria , biology , enzyme , telecommunications , computer science , genetics
In this paper we report the isolation and characterization of an anaerobic enrichment culture as well as of a Rhodococcus sp. strain 2 capable of degrading 3,4‐dihaloanilines under nitrate reducing conditions. Using mass spectrometry several of the intermediates formed in the process of 3,4‐dichloroaniline conversion were identified. Most interesting is the observation of reductive deamination and the formation of 1,2‐dichlorobenzene as one of the intermediates. Using 19 F NMR and fluorinated 3,4‐dihaloaniline model substrates it was corroborated that reductive deamination of the anilines to give dihalobenzene intermediates represents a new initial step in the anaerobic microbial degradation of these halogenated anilines.