Open AccessExpression of anthrax lethal factor gene by osmolyte induction
Author(s) -
Singh Aparna,
Singh Samer,
Waheed Syed Mohsin,
Khandelwal Puneet,
Bhatnagar Rakesh
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11148.x
Subject(s) - anthrax toxin , osmolyte , bacillus anthracis , anthrax vaccines , recombinant dna , cytosol , biology , immunity , microbiology and biotechnology , chemistry , gene , biochemistry , immunology , bacteria , fusion protein , immune system , genetics , enzyme , dna vaccination
The anthrax toxin consists of protective antigen (PA), lethal factor (LF) and edema factor (EF). PA mediates the entry of LF and EF to the cytosol where they exert their effects. Although PA is the major component of the vaccines against anthrax, LF has also been found to play an important role in enhancing protective immunity. We have developed an osmolyte‐inducible LF expression system. The protein expression system contributed no additional amino acids to the recombinant LF making it suitable for the human vaccine trials.