Open AccessTranscriptional analysis of the recA gene in Streptomyces rimosus : identification of the new type of promoter
Author(s) -
Ahel Ivan,
Vujaklija Dušica,
Mikoč Andreja,
Gamulin Vera
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11121.x
Subject(s) - promoter , streptomyces coelicolor , biology , gene , transcription (linguistics) , streptomyces , genetics , consensus sequence , orfs , primer extension , microbiology and biotechnology , dna , caat box , open reading frame , gene expression , mutant , peptide sequence , rna , linguistics , philosophy , bacteria
Using primer‐extension analysis we identified two transcription start sites for the recA gene in Streptomyces rimosus . A longer, weak transcript is initiated from the distal SEP promoter that contains a Cheo box like sequence: GAAC‐N4‐ATTC. However, the major start site of transcription is a G at position −36 and this shorter transcript significantly increases in response to DNA damage by UV‐light. The −35 box (TTGTCA) and −10 box (TAGCGT) of the strong recA promoter are only 11 bp apart and this proximal promoter is almost identical to the strong, DNA damage‐inducible promoter of Mycobacterium tuberculosis recA gene. We inspected the Streptomyces coelicolor database and found this type of promoter in the upstream regions of many (potentially) UV‐inducible genes as well as some other genes/ORFs. Moreover, the DNA sequence between the predicted −35 and −10 boxes is also partially conserved. The consensus sequence for this new type of promoter in Streptomyces is: TTGTCA GTGGC‐N6‐ TAGggT .