Open AccessIsolation and characterisation of 8‐hydroxy‐3 Z ,5 Z ‐tetradecadienoic acid, a putative intermediate in Pichia guilliermondii γ‐decalactone biosynthesis from ricinoleic acid
Author(s) -
Iacazio G,
Martini D,
Faure B,
N'Guyen M.H.
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11109.x
Subject(s) - ricinoleic acid , biosynthesis , pichia , yeast , chemistry , stereochemistry , biochemistry , enzyme , pichia pastoris , gene , castor oil , recombinant dna
During a screening procedure for the discovery of a strong γ‐decalactone producer from ricinoleic acid, we observed that the yeast Pichia guilliermondii accumulated transiently 8‐hydroxy‐3 Z ,5 Z ‐tetradecadienoic acid 1 during γ‐decalactone biosynthesis in the stationary phase of growth. The structural elucidation of 1 was based on nuclear magnetic resonance, infrared, ultraviolet and gas chromatography–mass spectrometry experiments. The occurrence of 1 is discussed in relation with previously proposed γ‐decalactone biosynthetic pathways.