Open AccessAspergillus nidulans sod VI C1 mutation causes defects in cell wall biogenesis and protein secretion
Author(s) -
Lee HwanHee,
Park JeongSeok,
Chae Suhn Kee,
Maeng Pil Jae,
Park HeeMoon
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11090.x
Subject(s) - aspergillus nidulans , mutant , secretion , biochemistry , chitin synthase , saccharomyces cerevisiae , biology , mutation , mannose , extracellular , protease , cell wall , chitin , chemistry , enzyme , yeast , gene , chitosan
Growth at the restrictive temperature (42°C) of Aspergillus nidulans B120, carrying the conditional‐lethal mutation sod VI C1 , was partially improved by the addition of 1.0 M sorbitol to the medium. The mutant grown at 42°C, with osmotic stabilizer, showed abnormal hyphal morphology, a decrease in β‐1,3‐glucan synthase activity as well as cell wall sugar content, but an increase in chitin synthase activity and N ‐acetyl‐glucosamine content. The mutation also affected the secretion of extracellular protease. The temperature‐dependent osmo‐sensitive phenotype of a Saccharomyces cerevisiae α‐COP mutation can be rescued by the A. nidulans sod VI C + gene. These results indicate that the sod VI C1 mutation affects proper processing of secretory proteins destined for the surface of cells or beyond.