Open AccessIdentification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress
Author(s) -
Eun Rhee Jee,
Haeng Rhee Joon,
Youl Ryu Phil,
Ho Choi Sang
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11089.x
Subject(s) - vibrio vulnificus , operon , biology , gene , mutant , vibrio , lysine decarboxylase , enterobacteriaceae , microbiology and biotechnology , lysine , transposable element , bacteria , vibrionaceae , transposon mutagenesis , vibrio infections , genetics , amino acid , escherichia coli , biochemistry , enzyme , spermidine , cadaverine
By a transposon‐tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus . The deduced amino acid sequences of cadBA were 64–97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus , and that their contribution is dependent on prior exposure of cells to moderately acidic pH.