Open AccessNitrite reduction in Paracoccus sp. is affected by a novel plasmid pYR1
Author(s) -
Barak Yoram,
Yarmus Merav,
Shapira Roni,
Rijn Jaap
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11069.x
Subject(s) - plasmid , subcloning , extrachromosomal dna , nitrite , biology , microbiology and biotechnology , nitrate , chemistry , dna , genetics , biochemistry , ecology
Two relatively low‐copy plasmids of 9 and 16 kb were found to comprise the extrachromosomal DNA of a Paracoccus strain. Reduction of nitrate by plasmid‐cured cells resulted in a significant intermediate nitrite accumulation as compared to wild‐type cells. By examining nitrate reduction by transformants containing one of the two plasmids, it was found that nitrite accumulation was influenced by the 9.0‐kb plasmid, designated as pYR1. Subcloning analysis showed that a 1.8‐kb fragment of this plasmid affected nitrite accumulation. Sequence analysis of this fragment revealed the presence of five open reading frames. One of the six deduced proteins showed a strong homology to ABC transporters.