Open AccessGenetic and restriction analysis of the 16S–23S rDNA internal transcribed spacer regions of the acetic acid bacteria
Author(s) -
Trček Janja,
Teuber Michael
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11062.x
Subject(s) - haeiii , internal transcribed spacer , bacteria , biology , hpaii , spacer dna , restriction enzyme , genetics , gene , microbiology and biotechnology , ribosomal rna , polymerase chain reaction , restriction fragment length polymorphism , gene expression , dna methylation
The 16S–23S rDNA internal transcribed spacer regions of the acetic acid bacteria were sequenced and evaluated for molecular identification of these bacteria. All the sequenced spacers contained genes for tRNA Ile and tRNA Ala , and the antitermination element. The sequences revealed 56.8–78.3% similarity. By PCR amplification of the spacers from 57 strains of acetic acid bacteria, single products of similar sizes were produced. Digestion of the spacers by Hae III and Hpa II restriction enzymes resulted in 12 distinct groups of restriction types. All the restriction profiles obtained after analysis of microbial populations from vinegar matched one of the 12 groups.