Open AccessDiversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen
Author(s) -
Gong Jianhua,
Forster Robert J,
Yu Hai,
Chambers James R,
Sabour Parviz M,
Wheatcroft Roger,
Chen Shu
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11051.x
Subject(s) - biology , bacteria , microbiology and biotechnology , 16s ribosomal rna , phylogenetic tree , ribosomal rna , intestinal mucosa , terminal restriction fragment length polymorphism , population , restriction fragment length polymorphism , genetics , polymerase chain reaction , gene , medicine , demography , sociology
We reported the first attempt to describe mucosa‐associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram‐positive with low G+C. Fusobacterium prausnitzii and butyrate‐producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T‐RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture‐independent approach rather than a culture‐based approach.