Open AccessPhysical and genetic map of the Bacteroides fragilis YCH46 chromosome
Author(s) -
Kuwahara Tomomi,
Sarker Mahfuzur Rahman,
Ugai Hideyo,
Akimoto Shigeru,
Shaheduzzaman Syed Mohammed,
Nakayama Haruyuki,
Miki Tsuyoshi,
Ohnishi Yoshinari
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11050.x
Subject(s) - bacteroides fragilis , biology , chromosome , genetics , restriction map , circular bacterial chromosome , clone (java method) , gene mapping , physical mapping , gene , restriction fragment , pulsed field gel electrophoresis , microbiology and biotechnology , bacteria , genome , plasmid , genotype
The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 Not I, seven Asc I, and six I‐ Ceu I sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross‐Southern hybridization, partial restriction digestion, and two‐dimensional pulsed‐field gel electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map.