Open AccessPhylogeny of the filamentous bacterium Eikelboom Type 1851, and design and application of a 16S rRNA targeted oligonucleotide probe for its fluorescence in situ identification in activated sludge
Author(s) -
Beer Michael,
Seviour Elizabeth M,
Kong Yun,
Cunningham Mitchell,
Blackall Linda L,
Seviour Robert J
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11048.x
Subject(s) - 16s ribosomal rna , chloroflexi (class) , activated sludge , segmented filamentous bacteria , bacteria , biology , oligonucleotide , ribosomal rna , microbiology and biotechnology , fluorescence in situ hybridization , gene , proteobacteria , biochemistry , sewage treatment , genetics , engineering , chromosome , waste management
Micromanipulation was used to obtain an isolate (BEN 52) of Eikelboom Type 1851 from a bulking activated sludge plant. Its 16S rDNA sequence reveals its closest relative is ‘ Roseiflexus castenholzii ’, a member of the phylum ‘Chloroflexi’, class ‘Chloroflexi’, previously called the green non‐sulfur bacteria. The 16S rRNA targeted oligonucleotide probe designed for fluorescence in situ hybridisation against this sequence successfully identified filamentous bacteria with the morphological features of Type 1851 in activated sludge samples from plants in several countries and different operational configurations.