Open AccessDual cell wall/mitochondria localization of the ‘SUN’ family proteins
Author(s) -
Velours Gisèle,
Boucheron Catherine,
Ma Stéphen,
Camougrand Nadine
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11046.x
Subject(s) - mitochondrion , dual (grammatical number) , cell , microbiology and biotechnology , cell wall , biology , genetics , art , literature
The Saccharomyces cerevisiae SUN family gene products, namely Sim1p, Uth1p, Nca3p and Sun4p, show a high degree of homology among themselves and are closely related to β‐glucosidase of Candida wickerhamii ; however, these proteins do not bear such an activity. Dithiothreitol‐treatment of intact cells induces the release of Uth1p, Sun4p and Sim1p from the cell wall. These highly glycosylated proteins are thus non‐covalently bound to the cell wall. Two of them, Uth1p and Sun4p, have also been found in mitochondria. Sub‐localization experiments show that Uth1p is inserted in the outer mitochondrial membrane and that Sun4p is preferentially a matrix protein. The physiological significance of this double localization is discussed in relation to the roles of these proteins in different cellular processes, namely mitochondrial biogenesis and cell septation.