Open AccessDNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1
Author(s) -
Xu Xudong,
Kong Renqiu,
Bruijn Frans J.,
He Sheng Yang,
Murry Marcia A.,
Newman Thomas,
Wolk C.Peter
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11036.x
Subject(s) - frankia , plasmid , biology , strain (injury) , genetics , dna sequencing , dna , sequence (biology) , microbiology and biotechnology , bacteria , root nodule , symbiosis , anatomy
An 8551‐bp plasmid, pFQ11, from Frankia alni strain CpI1 was sequenced. Its sequence was found to be very similar to that presented for pFQ31 from strain ArI3. Six potential protein‐encoding open reading frames (ORFs) were identified, and transcriptional activity was shown within four of those regions of the plasmid by reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis. An earlier study reported that ORF E F of pFQ31, which is nearly identical to the 3′ 45% of ORF1 of pFQ11, is significantly similar to RepF. We found no such similarity. ORF2 and ORF3 predict products that are similar to a repressor protein and a partition protein, respectively. We found inverted repeats within and covering the start codon of ORF3; palindromic sequences and direct repeats between ORF3 and ORF4; and 3′ from ORF3, an AT‐rich sequence that extensively overlaps the promoter region of a uvrB homolog in strain ArI3.