Open AccessIdentification of lactoferrin‐binding proteins in Streptococcus dysgalactiae subsp. dysgalactiae and Streptococcus agalactiae isolated from cows with mastitis
Author(s) -
Park HeeMyung,
Almeida Raul A,
Oliver Stephen P
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11033.x
Subject(s) - streptococcus dysgalactiae , streptococcus agalactiae , lactoferrin , microbiology and biotechnology , biology , mastitis , streptococcaceae , gel electrophoresis , streptococcus , bacteria , genetics , antibiotics
Three strains of Streptococcus dysgalactiae subsp. dysgalactiae ( S. dysgalactiae ) and five strains of Streptococcus agalactiae were used to identify lactoferrin‐binding proteins (LBPs). LBPs from extracted surface proteins were detected by polyacrylamide gel electrophoresis and Western blotting. All strains of S. dysgalactiae evaluated had 52‐ and 74‐kDa protein bands. All strains of S. agalactiae evaluated had 52‐, 70‐ and 110‐kDa protein bands. In addition, a 45‐kDa band was detected in two of five S. agalactiae strains evaluated. This study demonstrated that S. dysgalactiae and S. agalactiae of bovine origin contain two and three major LBPs, respectively