Open AccessAnalysis of the Schwanniomyces occidentalis SWA2 gene promoter in Saccharomyces cerevisiae
Author(s) -
Carmona T.A.,
Jiménez A,
Fernández Lobato M
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11030.x
Subject(s) - saccharomyces cerevisiae , genetics , gene , promoter , biology , microbiology and biotechnology , gene expression
The effect of different carbon sources on the expression in Saccharomyces cerevisiae of the SWA2 α‐amylase gene from Schwanniomyces occidentalis was studied from constructs containing its 5′ region (−223 to +15), which were fused in‐frame to the lacZ gene coding sequence. Maximal expression was achieved with the non‐fermentable substrates ethanol and/or glycerol, whereas lower levels were found with maltose or galactose. In contrast, glucose repressed it, even in the presence of any of these other carbon sources. Deletion analyses of the −233 to −85 SWA2 promoter region permitted the identification of two fragments involved in both glucose repression and ethanol activation. A possible region required for cAMP regulation was localised. The SWA2 promoter contains a MIG1‐binding GC box whose deletion caused a five‐fold increase in the glucose‐repressed reporter expression. Despite this, expression of the SWA2 promoter was not MIG1 ‐dependent.