Open AccessIdentification and molecular characterisation of CmeB, a Campylobacter jejuni multidrug efflux pump
Author(s) -
Pumbwe Lilian,
Piddock Laura J.V.
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11007.x
Subject(s) - efflux , campylobacter jejuni , ethidium bromide , atp binding cassette transporter , biology , microbiology and biotechnology , mutant , insertional mutagenesis , tetracycline , chemistry , biochemistry , gene , transporter , genetics , bacteria , antibiotics , dna
A multidrug efflux pump gene ( cmeB ) was identified from the published Campylobacter jejuni genome sequence. Secondary structural analysis showed that the gene encoded a protein belonging to the resistance nodulation cell division (RND) family of efflux transporters. The gene was inactivated by insertional mutagenesis. Compared with the wild‐type strain (NCTC 11168), the resultant knockout strain (NCTC 11168‐ cmeB::kan r ) displayed increased susceptibility to a range of antibiotics including β‐lactams, fluoroquinolones, macrolides, chloramphenicol, tetracycline, ethidium bromide, the dye acridine orange and the detergent sodium dodecyl sulfate. Accumulation of ciprofloxacin was increased in the knockout mutant, but carbonyl cyanide m ‐chlorophenyl hydrazone, a proton motive force inhibitor, had less effect upon ciprofloxacin accumulation in the knockout mutant compared with NCTC 11168. These data show that the identified gene encodes an RND‐type multi‐substrate efflux transporter, which contributes to intrinsic resistance to a range of structurally unrelated compounds in C. jejuni . This efflux pump has been named CmeB (for C ampylobacter m ultidrug e fflux).