Open AccessCloning and characterization of a glycosyltransferase gene involved in the biosynthesis of anthracycline antibiotic β‐rhodomycin from Streptomyces violaceus
Author(s) -
Miyamoto Yuji,
Johdo Osamu,
Nagamatsu Yasunori,
Yoshimoto Akihiro
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11003.x
Subject(s) - biology , gene , gene cluster , glycosyltransferase , genetics , complementation , mutant
A glycosyltransferase gene, rhoG , involved in the biosynthesis of the anthracycline antibiotic β‐rhodomycin was isolated as a 4.1‐kb DNA fragment containing rhoG and its flanking region from Streptomyces violaceus by degenerate and inverse PCR. Sequencing analysis showed that rhoG was located in a gene cluster involved in the biosynthesis of the constitutive deoxysugar of β‐rhodomycin. The function of rhoG was verified by gene disruption, which was generated by replacing the internal 0.9‐kb region of S. violaceus chromosome with a fragment including the Sac I‐blunted region. The rhoG disruption resulted in complete loss of β‐rhodomycin productivity, along with the accumulation of a non‐glycosyl intermediate ?‐rhodomycinone. In addition, the complementation test demonstrated that rhoG restored β‐rhodomycin production in this gene disruptant. These results indicated that rhoG is the glycosyltransferase gene responsible for the glycosylation of ?‐rhodomycinone in β‐rhodomycin biosynthesis.