Open AccessIdentification of enteric pathogens by heat shock protein 60 kDa (HSP60) gene sequences
Author(s) -
Wong Rebecca S.Y.,
Chow Anthony W.
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb10994.x
Subject(s) - biology , campylobacter jejuni , microbiology and biotechnology , shigella , yersinia , hypervariable region , escherichia coli , salmonella , campylobacter , heat shock protein , yersinia enterocolitica , hsp60 , enterobacteriaceae , gene , bacteria , restriction enzyme , vibrio cholerae , polymerase chain reaction , genetics , hsp70
A highly specific and reproducible approach for the simultaneous detection of enteric pathogenic bacteria was developed using bacterial hsp60 gene and molecular biological tools. A single pair of universal primers was derived from the highly conserved sequence of hsp60 genes encompassing a 600‐bp hypervariable region. PCR amplification followed by either dot blot hybridization or restriction enzyme digestion performed on 38 enteric bacteria indicated that this approach could differentiate not only different genera such as Campylobacter , Yersinia and Vibrio , but also species that are closely related genetically, such as between C. jejuni and C. coli , or between Salmonella and Shigella or Escherichia coli .