Open AccessCopper and cadmium increase laccase activity in Pleurotus ostreatus
Author(s) -
Baldrian Petr,
Gabriel Jiřı́
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb10988.x
Subject(s) - laccase , pleurotus ostreatus , cadmium , chemistry , pleurotus , copper , enzyme assay , enzyme , food science , straw , nuclear chemistry , biochemistry , inorganic chemistry , mushroom , organic chemistry
Addition of copper (0.5–5 mM) or cadmium (1–5 mM) to the white rot fungus Pleurotus ostreatus cultivated in liquid nitrogen‐limited medium for 12 days increased the activity of laccase. The addition of 2 mM Cd led to an 18.5‐fold increase of activity, 1 mM Cu increased the activity eight‐fold. When added earlier than 12 days, the activation of laccase was delayed (Cu) or decreased (Cd). Ag, Hg, Pb, Zn, and H 2 O 2 decreased laccase activity. To study the effect on native enzymes, purified laccase was incubated with Cd, Cu, and Hg. The addition of Hg decreased the activity of laccase immediately and reduced the temporal stability of the enzyme, while the addition of Cu (0.05–50 mM) increased both enzyme activity and stability. Laccase extracted at different stages of straw colonisation differed in its response to heavy metals.