Open AccessElectrophoretic karyotype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes
Author(s) -
Chávez Renato,
Fierro Francisco,
Gordillo Felipe,
Francisco Martı́n Juan,
Eyzaguirre Jaime
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10976.x
Subject(s) - biology , esterase , chromosome , genetics , gene , penicillium , genome , karyotype , xylanase , microbiology and biotechnology , genome size , southern blot , biochemistry , enzyme
The electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum has been resolved. Using contour‐clamped homogeneous electric field gel electrophoresis, five chromosomal bands were separated, with estimated sizes of 7.1, 5.2, 3.7, 2.9 and 2.3 Mbp, giving a total genome size of 21.2 Mbp. To our knowledge, this is the smallest Penicillium genome determined so far. By Southern blots and using homologous probes, the chromosomal location of five xylanolytic genes from P. purpurogenum was determined: axeI (acetyl xylan esterase I), xynB (endoxylanase B) and abf1 (arabinofuranosidase 1) in chromosome I, xynA (endoxylanase A) in chromosome II, and axeII (acetyl xylan esterase II) in chromosome III. This is the first study where the location of xylanase genes in a Penicillium genome has been established.