Open AccessThe genetic dependence of RecBCD‐Gam mediated double strand end repair in Escherichia coli
Author(s) -
Paškvan Ivan,
SalajŠmic Erika,
IvančićBaće Ivana,
Zahradka Ksenija,
Trgovčević Željko,
BrčićKostić Krunoslav
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10964.x
Subject(s) - recbcd , lysogenic cycle , escherichia coli , dna repair , helicase , biology , mutant , lysogen , genetics , radioresistance , lambda phage , dna , microbiology and biotechnology , gene , bacteriophage , cell culture , rna
Abstract The repair of double strand breaks after γ‐irradiation in wild‐type Escherichia coli lysogenic for λ cI 857 red3 is more efficient when λ Gam protein is present. This phenomenon, called gam dependent radioresistance, requires the interaction of RecBCD enzyme and Gam protein. We compared cell survival after γ‐irradiation in wild‐type and mutant lysogens with and without induction of Gam by transient heat treatment of the cells (6 min, 42°C). The main conclusions are: (1) the RecBCD‐Gam pathway of recombination repair is similar but not equivalent to RecBCD‡, a pathway operating in recD mutants; (2) the RecBCD‐Gam pathway is dependent on recJ , recQ and recN gene products and it is proposed that the RecBCD‐Gam complex has ability to load RecA protein onto single strand DNA.