Open AccessIsolation and characterization of a new poly(3‐hydroxybutyrate)‐degrading, denitrifying bacterium from activated sludge
Author(s) -
Tabrez Khan Shams,
Hiraishi Akira
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10957.x
Subject(s) - denitrifying bacteria , denitrification , activated sludge , nitrite , aerobic denitrification , chemistry , nitrate , proteobacteria , bacteria , microbiology and biotechnology , nuclear chemistry , food science , biology , biochemistry , 16s ribosomal rna , organic chemistry , nitrogen , waste management , sewage treatment , gene , engineering , genetics
A new denitrifying chemoorganotrophic bacterium capable of aerobic and anaerobic degradation of poly(3‐hydroxybutyrate) (PHB) and poly(3‐hydroxybutyrate‐co‐hydroxyvalerate) (PHBV) was isolated from activated sludge. A phylogenetic analysis based on 16S rDNA sequences indicated that the new isolate is a member of the β subclass of the Proteobacteria and represents a distinct line of descent within the family Comamonadaceae. During denitrifying growth with 3‐hydroxybutyrate, PHB, or PHBV as the sole carbon source, the isolate reduced nitrate to N 2 without appreciable accumulation of nitrite and nitrous oxide as intermediate products. Kinetic analyses of the denitrification with different grades of PHBV indicated that approximately 0.7 g of PHBV was required to reduce 1 g of NO 3 − . A high denitrification rate (19 mg N‐NO 3 − removed h −1 g −1 dry wt of cells) was found with PHBV as the electron donor.