Open AccessVacuolar H + ‐ATPase, but not mitochondrial F 1 F 0 ‐ATPase, is required for aluminum resistance in Saccharomyces cerevisiae
Author(s) -
Hamilton Christie A,
Good Allen G,
Taylor Gregory J
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10953.x
Subject(s) - saccharomyces cerevisiae , atpase , biology , mutant , biochemistry , yeast , vacuole , mitochondrion , enzyme , v atpase , microbiology and biotechnology , gene , cytoplasm
It was recently shown that vacuolar ATPase and mitochondrial F 1 F 0 ‐ATPase activities are induced by aluminum (Al) in an Al‐resistant cultivar of wheat, suggesting that induction of these enzymes could be an adaptive trait involved in Al resistance. To test this hypothesis, we used the Saccharomyces cerevisiae model system. In yeast, unlike wheat, the activity, transcript and protein levels of mitochondrial F 1 F 0 ‐ATPase, but not vacuolar ATPase, are induced by Al, while plasma membrane P‐ATPase activity is inhibited. However, yeast vacuolar ATPase mutant strains are hypersensitive to Al, while F 1 F 0 ‐ATPase mutant strains exhibit wild‐type growth. These data suggest that vacuolar ATPase activity is involved in Al resistance, with ATP required for this activity supplied by mitochondrial F 1 F 0 ‐ATPase or fermentation.