Open AccessInterspecies H 2 transfer in cellulose degradation between fibrolytic bacteria and H 2 ‐utilizing microorganisms from the human colon
Author(s) -
Robert Céline,
Del'Homme Christophe,
BernalierDonadille Annick
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10949.x
Subject(s) - cellulose , microorganism , methanogen , bacteria , biology , fermentation , biochemistry , autotroph , metabolism , strain (injury) , microbiology and biotechnology , chemistry , food science , genetics , anatomy
Interspecies H 2 transfer between two newly isolated fibrolytic strains (18P13 and 18P16) and H 2 ‐utilizing methanogen or acetogen from the human colon was investigated during in vitro cellulose degradation. Both H 2 ‐consuming microorganisms utilized efficiently H 2 produced from cellulose fermentation by the fibrolytic species. H 2 utilization by Methanobrevibacter smithii did not change the metabolism and the cellulolytic activity of strain 18P16 whereas it induced a metabolic shift in strain 18P13. However, this metabolic shift was not associated with enhancement of cellulose degradation. In contrast, an increase in cellulose breakdown was observed when strain 18P13 was cultivated with Ruminococcus hydrogenotrophicus . This stimulating effect could be attributed to both the autotrophic and the heterotrophic metabolism of the acetogen in the coculture.