Open AccessEarly translocation of acid‐adapted Listeria monocytogenes during enteric infection in TNF/LTα−/− mice
Author(s) -
Fontan Elisabeth,
SaklaniJusforgues Helène,
Goossens Pierre L
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10944.x
Subject(s) - listeria monocytogenes , mesenteric lymph nodes , spleen , caecum , microbiology and biotechnology , tumor necrosis factor alpha , chromosomal translocation , biology , listeria , inoculation , lymph , immunology , bacteria , medicine , pathology , biochemistry , genetics , gene
TNF/LTα deficient mice are devoid of Peyer's patches and lack mesenteric lymph nodes. Translocation, especially in the early steps after intragastric delivery of Listeria monocytogenes , has been explored in this study, and the role of TNFα has been addressed. We showed that L. monocytogenes translocation occurred at least as efficiently in TNF/LTα−/− mice as in TNF/LTα+/+ littermates. Even very low inocula (2.7×10 4 cfu) could initiate infection in the TNF/LTα deficient mice. Early kinetics of dissemination to the spleen and liver were similar, L. monocytogenes reaching these organs at 8 h post inoculation. However, a 10‐fold higher bacterial load was observed at this early time point in the TNF/LTα deficient mice. rTNF pretreatment (4 h before intragastric inoculation) had no effect on the L. monocytogenes associated with the caecum–colon walls at 10 h after inoculation, although bacterial levels in the caecum–colon lumen and in spleen and liver were already controlled.