Open AccessRapid orientated cloning in a shuttle vector allowing modulated gene expression in Bacillus subtilis
Author(s) -
Joseph Pascale,
Fantino JeanRaphael,
Herbaud MarieLaure,
Denizot François
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10930.x
Subject(s) - bacillus subtilis , shuttle vector , cloning (programming) , vector (molecular biology) , ribosomal binding site , expression vector , overproduction , gene , ribosome , biology , multiple cloning site , microbiology and biotechnology , genetics , computational biology , translation (biology) , messenger rna , bacteria , recombinant dna , computer science , rna , programming language
An expression vector for systematic protein overproduction in Bacillus subtilis has been constructed. It derives from pDG148 and combines the main property of this vector, i.e. conditional expression of the gene in response to isopropylβ‐ d ‐thiogalactopyranoside, with (i) rapid orientated cloning by a ligation independent procedure and (ii) a ribosome binding site of high translational efficiency. When used for overproduction of several proteins in B. subtilis , this vector gave good levels of protein synthesis.