Open AccessUra‐status‐dependent adhesion of Candida albicans mutants
Author(s) -
Bain Judith M,
Stubberfield Colin,
Gow Neil A.R.
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10905.x
Subject(s) - candida albicans , mutant , microbiology and biotechnology , adhesion , chemistry , biology , biochemistry , gene , organic chemistry
Gene disruptions in the diploid opportunistic human fungal pathogen Candida albicans are usually created using multiple rounds of targeted integration called the ‘ura‐blaster’ method. Resulting heterozygous and homozygous null mutants can be auxotrophic (Ura − ) or prototrophic (Ura + ) for uracil biosynthesis. Here we demonstrate that the Ura‐status of otherwise isogenic mutants affected the adhesion of C. albicans . Moreover the effect of Ura‐status on adhesion was also dependent on the null mutant background, the nature of the underlying surface and the carbon source for growth. Therefore the Ura‐status is not neutral in determining adhesive properties of C. albicans mutants that are generated via the ura‐blaster protocol.