Open AccessContribution of flagella and invasion proteins to pathogenesis of Salmonella enterica serovar enteritidis in chicks
Author(s) -
Parker Craig T,
GuardPetter Jean
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10899.x
Subject(s) - flagellin , microbiology and biotechnology , salmonella , salmonella enteritidis , salmonella enterica , mutant , biology , virulence , flagellum , serotype , wild type , bacteria , gene , genetics
To explore the relative contribution that flagella and Salmonella invasion proteins make to the virulence of Salmonella enteritidis in poultry, 20‐day‐old chicks were challenged orally and by subcutaneous injection with wild‐type strain SE‐HCD, two non‐flagellated mutants ( fliC ::Tn 10 mutant and flhD ::Tn 10 mutant) and two Salmonella invasion protein insertion mutants ( sipD and iacP ). When injected subcutaneously, wild‐type SE‐HCD was the only strain to cause substantial mortality and morbidity and to grow well in organs. The flhD mutant of SE‐HCD was invasive when given orally, whereas wild‐type SE‐HCD and the fliC mutant were significantly attenuated. Salmonella invasion protein mutants were not invasive by either route. These results suggest that temporary suppression of Class I regulators of flagellin biosynthesis may aid oral infection in poultry.