Open AccessProteins of the lactococcin A secretion system: lcnD encodes two in‐frame proteins
Author(s) -
Varcamonti Mario,
Nicastro Giovanna,
Venema Gerard,
Kok Jan
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10894.x
Subject(s) - lactococcus lactis , secretion , polyclonal antibodies , bacteriocin , secretory protein , antibody , biology , microbiology and biotechnology , biochemistry , chemistry , bacteria , genetics , lactic acid
Polyclonal antibodies were raised against LcnC and LcnD proteins of the Lactococcus lactis bacteriocin lactococcin A secretory system to examine their cellular location and interaction. Two major reacting bands were detected by Western immunoblot with the anti‐LcnD antibody: one of 52 kDa (LcnD) and another of 45 kDa, called here LcnD*. LcnD* was still detectable after removing the AUG start codon for LcnD. Chemical cross‐linking analyses of membrane fractions of L. lactis cells expressing the LcnC/D secretion machinery were performed. Our results indicate that LcnD is present in the secretion machinery complex as a dimer and is able to interact with LcnD* and LcnC.