Open AccessGene transfer using human papillomavirus pseudovirions varies according to virus genotype and requires cell surface heparan sulfate
Author(s) -
Combita Alba Lucia,
Touzé Antoine,
Bousarghin Latifa,
Sizaret PierreYves,
Muñoz Nubia,
Coursaget Pierre
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10883.x
Subject(s) - heparan sulfate , biology , gene , heterologous , plasmid , virology , gene transfer , virus , dna , microbiology and biotechnology , capsid , transfection , in vitro , cell , genetics
Artificial viruses consisting of DNA plasmid packaged in vitro into virus‐like particles (VLPs) are new vehicles for gene transfer. We therefore investigated the ability of nine human papillomavirus (HPV) VLPs to interact with heterologous DNA and transfer genes. HPV 16, 18, 31, 33, 39, 45, 58, 59, and 68 VLPs were able to bind heterologous DNA and to transfer genes into Cos‐7 cells. Inhibition of gene transfer by preincubation of the pseudovirions with heparin confirmed that heparan sulfate on the cell surface plays a role as cell receptor for HPVs. As HPV neutralizing antibodies are mainly type‐specific, gene transfer with different HPV pseudovirions offers the possibility of their sequential use in vivo for a greater efficacy.