Open AccessPhosphatidylinositol‐specific phospholipase C activity in Lactobacillus rhamnosus with capacity to translocate
Author(s) -
Rodriguez Ana V,
Baigorı́ Mario D,
Alvarez Susana,
Castro Guillermo R,
Oliver Guillermo
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10858.x
Subject(s) - lactobacillus rhamnosus , phosphatidylinositol , phospholipase c , microbiology and biotechnology , bacteria , lactic acid , biology , lactobacillus , biochemistry , kinase , enzyme , genetics
Phosphatidylinositol‐specific phospholipase C (PI‐PLC) activity was investigated in 25 different lactic acid bacteria (LAB) strains belonging to the genera Lactobacillus , Weisella , and Enterococcus . PI‐PLC activity was detected in 44% of the strains studied in culture medium without carbon source. From the PI‐PLC positive strains, Lactobacillus rhamnosus ATCC 7469 was selected for translocation studies. Healthy mice were orally administered with a daily dose of 2.0×10 9 of viable L. rhamnosus suspension. Viable bacteria were detected in liver and spleen of mice fed with LAB for 7 days. Bacterial colonies isolated from liver were biochemically characterized, and further subjected to randomly amplified polymorphic DNA. Amplification patterns of five strains displayed identical profiles to L. rhamnosus . PI‐PLC activity was determined in the strains recovered from liver.