Open AccessNa + ‐driven multidrug efflux pump VcmA from Vibrio cholerae non‐O1, a non‐halophilic bacterium
Author(s) -
Huda M.Nazmul,
Morita Yuji,
Kuroda Teruo,
Mizushima Tohru,
Tsuchiya Tomofusa
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10847.x
Subject(s) - ethidium bromide , efflux , vibrio cholerae , acriflavine , kanamycin , nalidixic acid , microbiology and biotechnology , ofloxacin , vibrionaceae , chemistry , escherichia coli , antiporter , biology , ciprofloxacin , bacteria , dna , biochemistry , gene , genetics , antibiotics , membrane
A chromosomal DNA fragment from Vibrio cholerae non‐O1 containing a drug resistance determinant was cloned and sequenced. The deduced amino acid sequence suggested that the determinant gene encodes a multidrug efflux pump. We designated the pump VcmA. Escherichia coli cells transformed with a plasmid carrying the vcmA gene showed increased resistance against norfloxacin, ciprofloxacin, ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium bromide, 4′,6‐diamidino‐2‐phenylindole dihydrochloride, Hoechst 33342 and acriflavine. Na + (or Li + )‐dependent efflux of ethidium bromide was detected in transformant cells. Efflux of Na + , elicited by ethidium bromide, was observed from transformant cells. Thus, we concluded that the VcmA is a Na + /drug antiporter.