Open AccessCloning and sequencing of the gene, fmtC , which affects oxacillin resistance in methicillin‐resistant Staphylococcus aureus
Author(s) -
Komatsuzawa Hitoshi,
Ohta Kouji,
Fujiwara Tamaki,
Choi Gil H,
Labischinski Harald,
Sugai Motoyuki
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10819.x
Subject(s) - fosfomycin , microbiology and biotechnology , staphylococcus aureus , mutant , bacitracin , methicillin resistant staphylococcus aureus , gene , biology , antibiotics , chemistry , genetics , bacteria
Two Tn 551 insertional mutants with reduced methicillin resistance were isolated from methicillin‐resistant Staphylococcus aureus KSA8. These two mutants showed increased susceptibility to β‐lactam antibiotics and bacitracin, but not to fosfomycin and vancomycin. Tn 551 in these mutants was inserted into the same gene, termed fmtC . The fmtC gene has an open reading frame of 840 amino acid residues with an estimated molecular mass of 96.9 kDa. The N‐terminal half of the deduced FmtC protein is very hydrophobic, implying that this protein is a membrane‐associated protein.