Open AccessExpression of the Pasteurella multocida ompH gene is negatively regulated by the Fur protein
Author(s) -
Bosch Montserrat,
Tarragó Raul,
Garrido Ma Elena,
Campoy Susana,
Fernández de Henestrosa Antonio R.,
Pérez de Rozas Ana M.,
Badiola Ignacio,
Barbé Jordi
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10817.x
Subject(s) - pasteurella multocida , biology , complementation , mutant , gene , microbiology and biotechnology , bacterial outer membrane , haemophilus influenzae , escherichia coli , groel , gene product , gene expression , genetics , bacteria , antibiotics
The fur gene of Pasteurella multocida has been cloned by complementation of an Escherichia coli fur mutant. The P. multocida fur gene, which encodes a predicted protein of 147 amino acids, displaying the highest identity (89%) with the same protein of Haemophilus influenzae , is negatively regulated by its own product. By construction of a P. multocida fur mutant, it has been demonstrated that the ompH gene, encoding a major structural protein of the outer membrane, presenting high antigenicity power, is negatively regulated by iron and glucose. Furthermore, wild‐type and fur ‐defective cells of P. multocida show the same level of virulence.